Langbein, Wolfgang  ORCID: https://orcid.org/0000-0001-9786-1023, Regan, David ORCID: https://orcid.org/0000-0003-0420-3481, Pope, Iestyn ORCID: https://orcid.org/0000-0002-4104-0389 and Borri, Paola ORCID: https://orcid.org/0000-0002-7873-3314
2018.
Heterodyne dual-polarization epi-detected CARS microscopy for chemical and topographic imaging of interfaces.
APL Photonics
3
, 092402.
10.1063/1.5027256
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Abstract
Coherent Raman Scattering (CRS) has emerged in the last decade as a powerful multiphoton microscopy technique offering chemically-specific label-free imaging in real time with high three-dimensional spatial resolution. Many technical realizations of CRS microscopy have been proposed to remove, suppress, or account for the non-resonant background in the nonlinear susceptibility which complicates spectral analysis and reduces image contrast. Here, we demonstrate coherent anti-Stokes Raman scattering microscopy using a dual-polarization balanced heterodyne detection in epi-geometry (eH-CARS), providing background-free chemically-specific image contrast for nanoparticles and interfaces, shot-noise limited detection, and phase sensitivity. We show the sensitivity and selectivity of eH-CARS in comparison with forward CARS and stimulated Raman scattering (SRS) on polystyrene beads in agarose gel. As an important biologically-relevant application, we demonstrate eH-CARS imaging of individual lipid bilayers with high contrast and topographic sensitivity.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Biosciences Schools > Physics and Astronomy |
| Subjects: | Q Science > QC Physics |
| Publisher: | AIP Publishing |
| ISSN: | 2378-0967 |
| Date of First Compliant Deposit: | 6 July 2018 |
| Date of Acceptance: | 22 May 2018 |
| Last Modified: | 07 Nov 2024 19:30 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/113008 |
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