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Heterodyne dual-polarization epi-detected CARS microscopy for chemical and topographic imaging of interfaces

Langbein, Wolfgang ORCID:, Regan, David ORCID:, Pope, Iestyn ORCID: and Borri, Paola ORCID: 2018. Heterodyne dual-polarization epi-detected CARS microscopy for chemical and topographic imaging of interfaces. APL Photonics 3 , 092402. 10.1063/1.5027256

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Coherent Raman Scattering (CRS) has emerged in the last decade as a powerful multiphoton microscopy technique offering chemically-specific label-free imaging in real time with high three-dimensional spatial resolution. Many technical realizations of CRS microscopy have been proposed to remove, suppress, or account for the non-resonant background in the nonlinear susceptibility which complicates spectral analysis and reduces image contrast. Here, we demonstrate coherent anti-Stokes Raman scattering microscopy using a dual-polarization balanced heterodyne detection in epi-geometry (eH-CARS), providing background-free chemically-specific image contrast for nanoparticles and interfaces, shot-noise limited detection, and phase sensitivity. We show the sensitivity and selectivity of eH-CARS in comparison with forward CARS and stimulated Raman scattering (SRS) on polystyrene beads in agarose gel. As an important biologically-relevant application, we demonstrate eH-CARS imaging of individual lipid bilayers with high contrast and topographic sensitivity.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Physics and Astronomy
Subjects: Q Science > QC Physics
Publisher: AIP Publishing
ISSN: 2378-0967
Date of First Compliant Deposit: 6 July 2018
Date of Acceptance: 22 May 2018
Last Modified: 08 Apr 2024 10:03

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