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Temporal profiling of the coding and noncoding murine cytomegalovirus transcriptomes

Lacaze, P., Forster, T., Ross, A., Kerr, L. E., Salvo-Chirnside, E., Lisnic, V. J., Lopez-Campos, G. H., Garcia-Ramirez, J. J., Messerle, M., Trgovcich, J., Angulo, A. and Ghazal, P. ORCID: 2011. Temporal profiling of the coding and noncoding murine cytomegalovirus transcriptomes. Journal of Virology 85 (12) , pp. 6065-6076. 10.1128/JVI.02341-10

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The global transcriptional program of murine cytomegalovirus (MCMV), involving coding, noncoding, and antisense transcription, remains unknown. Here we report an oligonucleotide custom microarray platform capable of measuring both coding and noncoding transcription on a genome-wide scale. By profiling MCMV wild-type and immediate-early mutant strains in fibroblasts, we found rapid activation of the transcriptome by 6.5 h postinfection, with absolute dependency on ie3, but not ie1 or ie2, for genomic programming of viral gene expression. Evidence is also presented to show, for the first time, genome-wide noncoding and bidirectional transcription at late stages of MCMV infection.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: American Society for Microbiology
ISSN: 0022-538X
Date of Acceptance: 28 March 2011
Last Modified: 23 Oct 2022 14:13

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