Connolly, Katherine D.  ORCID: https://orcid.org/0000-0003-1074-1710, Wadey, Rebecca M., Mathew, Donna, Johnson, Errin, Rees, D. Aled ORCID: https://orcid.org/0000-0002-1165-9092 and James, Philip E..
2018.
Evidence for adipocyte-derived extracellular vesicles in the human circulation.
Endocrinology
159
(9)
, pp. 3259-3267.
10.1210/en.2018-00266
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Abstract
Adipocyte-derived extracellular vesicles (EVs) may serve as novel endocrine mediators of adipose tissue and impact upon vascular health. However, it is unclear whether adipocyte-derived EVs are present in the human circulation. Therefore, the purpose of this study was to seek evidence for the presence of adipocyte-derived EVs in circulating plasma. Size exclusion chromatography of platelet-free plasma identified fractions 5-10 as containing EVs by a peak in particle concentration, which corresponded with the presence of EV and adipocyte proteins. Pooling fractions 5-10 and subjecting to ultracentrifugation yielded a plasma EV sample, as verified by transmission electron microscopy (TEM) showing EV structures and Western blotting for EV (e.g. CD9 and Alix) and adipocyte markers. Magnetic beads and a solid phase assay were used to deplete the EV sample of the four major families of circulating EVs: platelet-, leukocyte-, endothelial- and erythrocyte-derived EVs. Post-depletion samples from both techniques contained EV structures as visualized by TEM, as well as CD9, Alix and classic adipocyte proteins. Post-depletion samples also contained a range of other adipocyte proteins from an adipokine array. Adipocyte proteins and adipokines are expressed in optimally processed plasma EV samples, suggesting that adipocyte-derived EVs are secreted into the human circulation.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine Research Institutes & Centres > MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG) |
| Publisher: | Oxford University Press |
| ISSN: | 0013-7227 |
| Date of First Compliant Deposit: | 12 July 2018 |
| Date of Acceptance: | 10 July 2018 |
| Last Modified: | 18 Nov 2024 16:15 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/113164 |
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