Enhancerless cytomegalovirus Is capable of establishing a low-level maintenance infection in severely immunodeficient host tissues but fails in exponential growth

Podlech, J., Pintea, R., Kropp, K. A., Fink, A., Lemmermann, N. A. W., Erlach, K. C., Isern, E., Angulo, A., Ghazal, P. and Reddehase, M. J. 2011. Enhancerless cytomegalovirus Is capable of establishing a low-level maintenance infection in severely immunodeficient host tissues but fails in exponential growth. Journal of Virology 84 (12) , 024116. 10.1128/JVI.00419-10

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Abstract

The manipulation of ribosomal RNA (rRNA) extracted from E. coli cells by dielectrophoresis (DEP) has been demonstrated over the range of 3 kHz–50 MHz using interdigitated microelectrodes. Quantitative measurement using total internal reflection fluorescence microscopy of the time dependent collection indicated a positive DEP response characterized by a plateau between 3 kHz and 1 MHz followed by a decrease in response at higher frequencies. Negative DEP was observed above 9 MHz. The positive DEP response below 1 MHz is described by the Clausius–Mossotti model and corresponds to an induced dipole moment of 3300 D with a polarizability of 7.8×10 −32  F m 2 7.8×10−32 F m2 . The negative DEP response above 9 MHz indicates that the rRNA molecules exhibit a net moment of −250 D, to give an effective permittivity value of 78.5 ε 0 78.5 ε0 , close to that of the aqueous suspending medium, and a relatively small surface conductance value of ∼0.1 nS ∼0.1 nS . This suggests that our rRNA samples have a fairly open structure accessible to the surrounding water molecules, with counterions strongly bound to the charged phosphate groups in the rRNA backbone. These results are the first demonstration of DEP for fast capture and release of rRNA units, opening new opportunities for rRNA-based biosensing devices.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Schools > Medicine
Publisher:	American Society for Microbiology
ISSN:	0022-538X
Date of Acceptance:	1 June 2011
Last Modified:	20 Jul 2018 14:00
URI:	https://orca.cardiff.ac.uk/id/eprint/113219

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