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Solution state hybridization detection using time-resolved fluorescence anisotropy of quantum dot-DNA bioconjugates

Giraud, Gerard, Schulze, Holger, Bachmann, Till T., Campbell, Colin J., Mount, Andrew R., Ghazal, Peter, Khondoker, Mizanur R., Ember, Stuart W.J., Ciani, Ilenia, Tlili, Chaker, Walton, Anthony J., Terry, Jonathan G. and Crain, Jason 2009. Solution state hybridization detection using time-resolved fluorescence anisotropy of quantum dot-DNA bioconjugates. Chemical Physics Letters 484 (4-6) , pp. 309-314. 10.1016/j.cplett.2009.11.032

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Abstract

In this Letter, we demonstrate the application of time-resolved fluorescence anisotropy measurements to detect solution state hybridization of streptavidin conjugate (CdSe)ZnS quantum dots (QD). The study was performed on samples containing 10 nM QD incubated with 800 nM DNA. We show that the rotational correlation time of QD–DNA constructs increases significantly upon hybridization with values of 330 ns (QD-ssDNA) and 1.3 μs (QD-dsDNA), corresponding to a diameter of 14 nm and 23 nm respectively. The present study opens a new modality for hybridization detection using quantum dots.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Medicine
Publisher: Elsevier
ISSN: 0009-2614
Date of Acceptance: 13 November 2009
Last Modified: 19 Jul 2018 08:30
URI: https://orca.cardiff.ac.uk/id/eprint/113224

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