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DNA Nanoswitch as a Biosensor

Buck, Amy H., Campbell, Colin J., Dickinson, Paul, Mountford, Christopher P., Stoquert, Hélène C., Terry, Jonathan G., Evans, Stuart A. G., Keane, Lorraine M., Su, Tsueu-Ju, Mount, Andrew R., Walton, Anthony J., Beattie, John S., Crain, Jason and Ghazal, Peter ORCID: 2007. DNA Nanoswitch as a Biosensor. Analytical Chemistry 79 (12) , pp. 4724-4728. 10.1021/ac070251r

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We present a new type of DNA switch, based on the Holliday junction, that uses a combination of binding and conformational switching to enable specific label-free detection of DNA and RNA. We show that a single RNA oligonucleotide species can be detected in a complex mixture of extracted cellular RNA and demonstrate that by exploiting different aspects of the switch characteristics we can achieve 30-fold discrimination between single-nucleotide mismatches in a DNA oligonucleotide.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: American Chemical Society
ISSN: 0003-2700
Last Modified: 23 Oct 2022 14:18

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