Sphingomyelinase D from loxosceles laeta venom induces the expression of MMP7 in human keratinocytes: contribution to dermonecrosis

Correa, Mara A., Okamoto, Cinthya K., Goncalves-de-Andrade, Rute M., van den Berg, Carmen W. and Tambourgi, Denise V. 2016. Sphingomyelinase D from loxosceles laeta venom induces the expression of MMP7 in human keratinocytes: contribution to dermonecrosis. PLoS ONE 11 (4) , e0153090. 10.1371/journal.pone.0153090

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Abstract

Envenomation by Loxosceles spider is characterized by the development of dermonecrosis. In previous studies, we have demonstrated that increased expression/secretion of matrix metalloproteinases 2 and 9, induced by Loxosceles intermedia venom Class 2 SMases D (the main toxin in the spider venom), contribute to the development of cutaneous loxoscelism. In the present study we show that the more potent venom containing the Class 1 SMase D from Loxosceles laeta, in addition to increasing the expression/secretion of MMP2 and MMP9, also stimulates the expression of MMP7 (Matrilysin-1), which was associated with keratinocyte cell death. Tetracycline, a matrix metalloproteinase inhibitor, prevented cell death and reduced MMPs expression. Considering that L. laeta venom is more potent at inducing dermonecrosis than L. intermedia venom, our results suggest that MMP7 may play an important role in the severity of dermonecrosis induced by L. laeta spider venom SMase D. In addition, the inhibition of MMPs by e.g. tetracyclines may be considered for the treatment of the cutaneous loxoscelism.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	Public Library of Science
ISSN:	1932-6203
Date of First Compliant Deposit:	8 August 2018
Date of Acceptance:	23 March 2016
Last Modified:	05 May 2023 02:33
URI:	https://orca.cardiff.ac.uk/id/eprint/113926

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