A modular assembly of spinal cord-like tissue allows targeted tissue repair in the transected spinal cord

Lai, Bi-Qin, Feng, Bo, Che, Ming-Tian, Wang, Lai-Jian, Cai, Song, Huang, Meng-Yao, Gu, Huai-Yu, Jiang, Bing, Ling, Eng-Ang, Li, Meng ORCID: https://orcid.org/0000-0002-4803-4643, Zeng, Xiang and Zeng, Yuan-Shan 2018. A modular assembly of spinal cord-like tissue allows targeted tissue repair in the transected spinal cord. Advanced Science 5 (9) , 1800261. 10.1002/advs.201800261

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Abstract

Tissue engineering–based neural construction holds promise in providing organoids with defined differentiation and therapeutic potentials. Here, a bioengineered transplantable spinal cord–like tissue (SCLT) is assembled in vitro by simulating the white matter and gray matter composition of the spinal cord using neural stem cell–based tissue engineering technique. Whether the organoid would execute targeted repair in injured spinal cord is evaluated. The integrated SCLT, assembled by white matter–like tissue (WMLT) module and gray matter–like tissue (GMLT) module, shares architectural, phenotypic, and functional similarities to the adult rat spinal cord. Organotypic coculturing with the dorsal root ganglion or muscle cells shows that the SCLT embraces spinal cord organogenesis potentials to establish connections with the targets, respectively. Transplantation of the SCLT into the transected spinal cord results in a significant motor function recovery of the paralyzed hind limbs in rats. Additionally, targeted spinal cord tissue repair is achieved by the modular design of SCLT, as evidenced by an increased remyelination in the WMLT area and an enlarged innervation in the GMLT area. More importantly, the pro‐regeneration milieu facilitates the formation of a neuronal relay by the donor neurons, allowing the conduction of descending and ascending neural inputs.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Medicine Neuroscience and Mental Health Research Institute (NMHRI)
Additional Information:	This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Publisher:	Wiley Open Access
ISSN:	2198-3844
Date of First Compliant Deposit:	31 August 2018
Date of Acceptance:	6 June 2018
Last Modified:	06 May 2023 04:15
URI:	https://orca.cardiff.ac.uk/id/eprint/114519

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