Activation of Endogenous Cdc42 Visualized in Living Cells

Nalbant, Perihan, Hahn, K.M., Hodgson, L. and Toutchkine, A. 2004. Activation of Endogenous Cdc42 Visualized in Living Cells. Science 305 (5690) , 1615 -1619. 10.1126/science.1100367

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Abstract

Signaling proteins are tightly regulated spatially and temporally to perform multiple functions. For Cdc42 and other guanosine triphosphatases, the subcellular location of activation is a critical determinant of cell behavior. However, current approaches are limited in their ability to examine the dynamics of Cdc42 activity in living cells. We report the development of a biosensor capable of visualizing the changing activation of endogenous, unlabeled Cdc42 in living cells. With the use of a dye that reports protein interactions, the biosensor revealed localized activation in the trans-Golgi apparatus, microtubule-dependent Cdc42 activation at the cell periphery, and activation kinetics precisely coordinated with cell extension and retraction.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
ISSN:	1095-9203
Last Modified:	12 Feb 2016 22:02
URI:	https://orca.cardiff.ac.uk/id/eprint/1163

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