Identification of clinical isolates of - Hemolytic Streptococci by 16S rRNA gene sequencing, matrix-assisted laser desorption ionization - time of flight mass spectrometry using MALDI biotyper, and conventional phenotypic methods: a comparison

Davies, A. P., Reid, M., Hadfield, S. J., Johnston, S., Mikhail, J., Harris, L. G., Jenkinson, H. F., Berry, N., Lewis, A. M., El-Bouri, K. and Mack, D. 2012. Identification of clinical isolates of - Hemolytic Streptococci by 16S rRNA gene sequencing, matrix-assisted laser desorption ionization - time of flight mass spectrometry using MALDI biotyper, and conventional phenotypic methods: a comparison. Journal of Clinical Microbiology 50 (12) , pp. 4087-4090. 10.1128/JCM.02387-12

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Abstract

Fifty-six α-hemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMérieux), and BD Phoenix (Becton, Dickinson). The gold standard for identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. The following percentages of isolates were correctly identified to the species level: MALDI Biotyper, 46%; BD Phoenix, 35%; and API 20 Strep, 26%.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	American Society for Microbiology
ISSN:	0095-1137
Date of Acceptance:	7 September 2012
Last Modified:	12 Nov 2018 14:00
URI:	https://orca.cardiff.ac.uk/id/eprint/116515

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