Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extraction and manipulation

Oberacker, Phil, Stepper, Peter, Bond, Donna, Hohn, Sven, Focken, Jule, Meyer, Vivien, Schelle, Luca, Sugrue, Victoria, Jeunen, Gert-Jan, Moser, Tim, Hore, Steven, von Meyenn, Ferdinand, Hipp, Katharina, Hore, Timothy and Jurkowski, Tomasz ORCID: https://orcid.org/0000-0002-2012-0240 2019. Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extraction and manipulation. PLoS Biology 17 (1) , e3000107. 10.1371/journal.pbio.3000107

Preview

PDF - Published Version Available under License Creative Commons Attribution. Download (1MB) | Preview

Abstract

Current molecular biology laboratories rely heavily on the purification and manipulation of nucleic acids. Yet, commonly used centrifuge- and column-based protocols require specialised equipment, often use toxic reagents and are not economically scalable or practical to use in a high-throughput manner. Although it has been known for some time that magnetic beads can provide an elegant answer to these issues, the development of open-source protocols based on beads has been limited. In this article, we provide step-by-step instructions for an easy synthesis of functionalised magnetic beads, and detailed protocols for their use in the high-throughput purification of plasmids, genomic DNA and total RNA from different sources, as well as environmental TNA and PCR amplicons. We also provide a bead-based protocol for bisulfite conversion, and size selection of DNA and RNA fragments. Comparison to other methods highlights the capability, versatility and extreme cost-effectiveness of using magnetic beads. These open source protocols and the associated webpage (https://bomb.bio) can serve as a platform for further protocol customisation and community engagement.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Biosciences
Publisher:	Public Library of Science
ISSN:	1545-7885
Date of First Compliant Deposit:	5 February 2019
Date of Acceptance:	10 January 2019
Last Modified:	06 May 2023 04:40
URI:	https://orca.cardiff.ac.uk/id/eprint/118958

Citation Data

Cited 123 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item