Bioluminescence of Vibrio fischeri: bacteria respond quickly and sensitively to pulsed microwave electric (but not magnetic) fields

Williams, Catrin F. ORCID: https://orcid.org/0000-0001-8619-2581, Geroni, Gilles M., Lloyd, David ORCID: https://orcid.org/0000-0002-5656-0571, Choi, Heungjae ORCID: https://orcid.org/0000-0003-1108-293X, Clark, Nicholas ORCID: https://orcid.org/0000-0002-5306-0993, Pirog, Antoine, Lees, Jonathan ORCID: https://orcid.org/0000-0002-6217-7552 and Porch, Adrian ORCID: https://orcid.org/0000-0001-5293-8883 2019. Bioluminescence of Vibrio fischeri: bacteria respond quickly and sensitively to pulsed microwave electric (but not magnetic) fields. Journal of Biomedical Optics 24 (5) , 051412. 10.1117/1.JBO.24.5.051412

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Abstract

Biological systems with intrinsic luminescent properties serve as powerful and noninvasive bioreporters for real-time and label-free monitoring of cell physiology. This study employs the bioluminescent marine bacterium <i>Vibrio fischeri</i> to investigate the effects of separated microwave electric (E) and magnetic (H) fields. Using a cylindrical TM₀₁₀ mode aluminum resonant cavity, designed to spatially separate E and H fields of a pulsed microwave (2.45 GHz) input, we sampled at 100-ms intervals the 490-nm emission of bioluminescence from suspensions of the <i>V. fischeri</i>. E-field exposure (at 4.24 and 13.4 kV/m) results in rapid and sensitive responses to 100-ms pulses. H-field excitation elicits no measurable responses, even at 100-fold higher power input levels (equivalent to 183 A/m). The observed effects on bacterial light output partially correlate with measured E-field-induced temperature increases. In conclusion, the endogenous bioluminescence of <i>V. fischeri</i> provides a sensitive and noninvasive method to assess the biological effects of microwave fields.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Engineering Biosciences
Publisher:	Society of Photo-optical Instrumentation Engineers (SPIE)
ISSN:	1083-3668
Date of First Compliant Deposit:	1 March 2019
Date of Acceptance:	21 January 2019
Last Modified:	05 May 2023 00:50
URI:	https://orca.cardiff.ac.uk/id/eprint/120130

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