Checkpoint kinases and the INO80 nucleosome remodeling complex enhance global chromatin mobility in response to DNA damage

Seeber, Andrew, Dion, Vincent ORCID: https://orcid.org/0000-0003-4953-7637 and Gasser, Susan M. 2013. Checkpoint kinases and the INO80 nucleosome remodeling complex enhance global chromatin mobility in response to DNA damage. Genes {&} Development 27 (18) , 1999--2008. 10.1101/gad.222992.113

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Abstract

Double-strand break repair by recombination requires a homology search. In yeast, induced breaks move significantly more than undamaged loci. To examine whether DNA damage provokes an increase in chromatin mobility generally, we tracked undamaged loci under DNA-damaging conditions. We found that the yeast checkpoint factors Mec1, Rad9, and Rad53 are required for genome-wide increases in chromatin mobility, but not the repair protein Rad51. Mec1 activation by targeted Ddc1/Ddc2 enhances chromatin mobility even in the absence of damage. Finally, the INO80 chromatin remodeler is shown to act downstream from Mec1 to increase chromatin mobility, highlighting an additional damage-related role of this nucleosome remodeling complex.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Medicine
Last Modified:	25 Oct 2022 13:36
URI:	https://orca.cardiff.ac.uk/id/eprint/120304

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