Karuna, Arnica, Masia, Francesco  ORCID: https://orcid.org/0000-0003-4958-410X, Wiltshire, Marie, Errington, Rachel ORCID: https://orcid.org/0000-0002-8016-4376, Borri, Paola ORCID: https://orcid.org/0000-0002-7873-3314 and Langbein, Wolfgang ORCID: https://orcid.org/0000-0001-9786-1023
2019.
Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering.
Presented at: SPIE BIOS,
San Francisco, California, US,
2-7 Feb 2019.
Multiphoton Microscopy in the Biomedical Sciences XIX.
Proceedings of SPIE.
Proceedings of SPIE
, vol.10882
Bellingham, Washington:
SPIE,
108821P.
10.1117/12.2510277
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Abstract
Quantitative determination of the chemical composition of unstained samples, non-invasively, with high three- dimensional spatio-temporal resolution, will accelerate progress in cell biology. The current state of the art in bioimaging is dominated by either chemically non-specific or invasive methods. In this work, we demonstrate label-free, non-invasive quantitative volumetric imaging of human osteosarcoma cells using coherent anti-Stokes Raman scattering microscopy. A data analysis method developed in-house was applied to represent the chemical composition of the cells as volumetric three-dimensional images indicating water, proteins, DNAP (mixture of DNA and proteins), and lipids, and to determine the dry masses of the organic components with picogram resolution.
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine Schools > Biosciences Schools > Physics and Astronomy |
| Publisher: | SPIE |
| ISBN: | 9781510624078 |
| ISSN: | 2410-9045 |
| Date of First Compliant Deposit: | 18 March 2019 |
| Last Modified: | 05 Oct 2024 01:06 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/120685 |
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