Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering

Karuna, Arnica, Masia, Francesco ORCID: https://orcid.org/0000-0003-4958-410X, Wiltshire, Marie, Errington, Rachel ORCID: https://orcid.org/0000-0002-8016-4376, Borri, Paola ORCID: https://orcid.org/0000-0002-7873-3314 and Langbein, Wolfgang ORCID: https://orcid.org/0000-0001-9786-1023 2019. Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering. Presented at: SPIE BIOS, San Francisco, California, US, 2-7 Feb 2019. Multiphoton Microscopy in the Biomedical Sciences XIX. Proceedings of SPIE. Proceedings of SPIE , vol.10882 Bellingham, Washington: SPIE, 108821P. 10.1117/12.2510277

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Abstract

Quantitative determination of the chemical composition of unstained samples, non-invasively, with high three- dimensional spatio-temporal resolution, will accelerate progress in cell biology. The current state of the art in bioimaging is dominated by either chemically non-specific or invasive methods. In this work, we demonstrate label-free, non-invasive quantitative volumetric imaging of human osteosarcoma cells using coherent anti-Stokes Raman scattering microscopy. A data analysis method developed in-house was applied to represent the chemical composition of the cells as volumetric three-dimensional images indicating water, proteins, DNAP (mixture of DNA and proteins), and lipids, and to determine the dry masses of the organic components with picogram resolution.

Item Type:	Conference or Workshop Item (Paper)
Date Type:	Publication
Status:	Published
Schools:	Medicine Biosciences Physics and Astronomy
Publisher:	SPIE
ISBN:	9781510624078
ISSN:	2410-9045
Date of First Compliant Deposit:	18 March 2019
Last Modified:	05 Oct 2024 01:06
URI:	https://orca.cardiff.ac.uk/id/eprint/120685

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