Measuring sub-nanometre thickness changes during phase transitions of supported lipid bilayers with quantitative differential interference contrast microscopy

Regan, David ORCID: https://orcid.org/0000-0003-0420-3481, Williams, Joseph, Masia, Francesco ORCID: https://orcid.org/0000-0003-4958-410X, Borri, Paola ORCID: https://orcid.org/0000-0002-7873-3314 and Langbein, Wolfgang ORCID: https://orcid.org/0000-0001-9786-1023 2019. Measuring sub-nanometre thickness changes during phase transitions of supported lipid bilayers with quantitative differential interference contrast microscopy. Presented at: SPIE BIOS, San Francisco, California, US, 2-7 Feb 2019. Quantitative Phase Imaging V. Proceedings of SPIE. Proceedings of SPIE , vol.10887 Bellingham, Washington: SPIE, 10.1117/12.2510461

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Abstract

Quantitative differential interference contrast (qDIC) microscopy is applied to the study of the main phase transition of dipentadecanoylphosphatidylcholine (DC15PC) supported lipid bilayers. We measure thickness changes of about 1nm occurring in the bilayer with sub-nanometre resolution and show how the presence of fluorescently labelled lipids, even at small concentrations, can broaden the phase transition.

Item Type:	Conference or Workshop Item (Paper)
Date Type:	Publication
Status:	Published
Schools:	Physics and Astronomy Biosciences
Publisher:	SPIE
ISBN:	9781510624177
ISSN:	0277-786X
Date of First Compliant Deposit:	18 March 2019
Last Modified:	06 Jan 2024 04:50
URI:	https://orca.cardiff.ac.uk/id/eprint/120688

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