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In vitro neuroprotective activities of two distinct probiotic consortia

Michael, D.R., Davies, T.S., Loxley, K.E., Allen, M.D., Good, M.A. ORCID:, Hughes, T.R. ORCID: and Plummer, S.F. 2019. In vitro neuroprotective activities of two distinct probiotic consortia. Beneficial Microbes 10 (4) , pp. 437-447. 10.3920/BM2018.0105

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Neurodegeneration has been linked to changes in the gut microbiota and this study compares the neuroprotective capability of two bacterial consortia, known as Lab4 and Lab4b, using the established SH-SY5Y neuronal cell model. Firstly, varying total antioxidant capacities (TAC) were identified in the intact cells from each consortia and their secreted metabolites, referred to as conditioned media (CM). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Crystal Violet (CV) assays of cell viability revealed that Lab4 CM and Lab4b CM could induce similar levels of proliferation in SH-SY5Y cells and, despite divergent TAC, possessed a comparable ability to protect undifferentiated and retinoic acid-differentiated cells from the cytotoxic actions of rotenone and undifferentiated cells from the cytotoxic actions of 1-methyl-4-phenylpyridinium iodide (MPP+). Lab4 CM and Lab4b CM also had the ability to attenuate rotenone-induced apoptosis and necrosis with Lab4b inducing the greater effect. Both consortia showed an analogous ability to attenuate intracellular reactive oxygen species accumulation in SH-SY5Y cells although the differential upregulation of genes encoding glutathione reductase and superoxide dismutase by Lab4 CM and Lab4b CM, respectively, implicates the involvement of consortia-specific antioxidative mechanisms of action. This study implicates Lab4 and Lab4b as potential neuroprotective agents and justifies their inclusion in further in vivo studies.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Psychology
Publisher: Wageningen Academic Publishers
ISSN: 1876-2883
Date of First Compliant Deposit: 19 March 2019
Date of Acceptance: 11 December 2018
Last Modified: 04 May 2023 08:25

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