Analysis of isothermal titration calorimetry data for complex interactions using I2CITC

Saeed, Ibrahim Q. and Buurma, Niklaas J. ORCID: https://orcid.org/0000-0003-0260-5057 2019. Analysis of isothermal titration calorimetry data for complex interactions using I2CITC. Ennifar, Eric, ed. Microcalorimetry of Biological Molecules, Vol. 1964. Methods in Molecular Biology, Humana Press, pp. 169-183. (10.1007/978-1-4939-9179-2_13)

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Abstract

I2CITC allows the analysis of isothermal titration calorimetry (ITC) data for complex coupled equilibria. Here we describe how, using I2CITC, ITC data for systems involving a self-aggregating ligand and a host offering one or two binding sites can be analyzed, how interaction models can be tested, and how confidence intervals for the optimized parameters can be determined.

Item Type:	Book Section
Date Type:	Published Online
Status:	Published
Schools:	Schools > Chemistry
Publisher:	Humana Press
ISBN:	9781493991785
ISSN:	1064-3745
Last Modified:	04 Nov 2022 12:09
URI:	https://orca.cardiff.ac.uk/id/eprint/122094

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