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The minimum open reading frame, AUG-stop, induces boron-dependent ribosome stalling and mRNA degradation

Tanaka, Mayuki, Sotta, Naoyuki, Yamazumi, Yusuke, Yamashita, Yui, Miwa, Kyoko, Murota, Katsunori, Chiba, Yukako, Hirai, Masami Yokota, Akiyama, Tetsu, Onouchi, Hitoshi, Naito, Satoshi and Fujiwara, Toru 2016. The minimum open reading frame, AUG-stop, induces boron-dependent ribosome stalling and mRNA degradation. Plant Cell 28 (11) , p. 2830. 10.1105/tpc.16.00481

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Abstract

Upstream open reading frames (uORFs) are often translated ahead of the main ORF of a gene and regulate gene expression, sometimes in a condition-dependent manner, but such a role for the minimum uORF (hereafter referred to as AUG-stop) in living organisms is currently unclear. Here, we show that AUG-stop plays an important role in the boron (B)-dependent regulation of NIP5;1, encoding a boric acid channel required for normal growth under low B conditions in Arabidopsis thaliana. High B enhanced ribosome stalling at AUG-stop, which was accompanied by the suppression of translation and mRNA degradation. This mRNA degradation was promoted by an upstream conserved sequence present near the 5′-edge of the stalled ribosome. Once ribosomes translate a uORF, reinitiation of translation must take place in order for the downstream ORF to be translated. Our results suggest that reinitiation of translation at the downstream NIP5;1 ORF is enhanced under low B conditions. A genome-wide analysis identified two additional B-responsive genes, SKU5 and the transcription factor gene ABS/NGAL1, which were regulated by B-dependent ribosome stalling through AUG-stop. This regulation was reproduced in both plant and animal transient expression and cell-free translation systems. These findings suggest that B-dependent AUG-stop-mediated regulation is common in eukaryotes.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: American Society of Plant Biologists
ISSN: 1040-4651
Date of Acceptance: 19 September 2016
Last Modified: 10 Nov 2021 02:23
URI: https://orca.cardiff.ac.uk/id/eprint/122322

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