The Moraxella adhesin UspA1 binds to its human CEACAM1 receptor by a deformable trimeric coiled-coil

Conners, Rebecca, Hill, Darryl J., Borodina, Elena, Agnew, Christopher, Daniell, Sarah J., Burton, Nicholas M., Sessions, Richard B., Clarke, Anthony R., Catto, Lucy E., Lammie, Donna, Wess, Timothy James, Brady, R. Leo and Virji, Mumtaz 2008. The Moraxella adhesin UspA1 binds to its human CEACAM1 receptor by a deformable trimeric coiled-coil. Embo Journal 27 (12) , pp. 1779-1789. 10.1038/emboj.2008.101

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Abstract

Moraxella catarrhalis is a ubiquitous human-specific bacterium commonly associated with upper and lower respiratory tract infections, including otitis media, sinusitis and chronic obstructive pulmonary disease. The bacterium uses an autotransporter protein UspA1 to target an important human cellular receptor carcinoembryonic antigen- related cell adhesion molecule 1 (CEACAM1). Using X-ray crystallography, we show that the CEACAM1 receptor- binding region of UspA1 unusually consists of an extended, rod-like left-handed trimeric coiled-coil. Mutagenesis and binding studies of UspA1 and the N-domain of CEACAM1 have been used to delineate the interacting surfaces between ligand and receptor and guide assembly of the complex. However, solution scattering, molecular modelling and electron microscopy analyses all indicate that significant bending of the UspA1 coiled-coil stalk also occurs. This explains how UspA1 can engage CEACAM1 at a site far distant from its head group, permitting closer proximity of the respective cell surfaces during infection.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Optometry and Vision Sciences
Subjects:	Q Science > QR Microbiology
Uncontrolled Keywords:	Adhesion molecules ; Bacterial adhesin ; Coiled-coil ; SAXS ; X-ray crystallography
Publisher:	Nature Publishing Group
ISSN:	0261-4189
Last Modified:	19 Mar 2016 22:20
URI:	https://orca.cardiff.ac.uk/id/eprint/12288

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