Long wavelength TCF-based fluorescent probe for the detection of alkaline phosphatase in live cells

Gwynne, Lauren, Sedgwick, Adam C., Gardiner, Jordan E., Williams, George T., Kim, Gyoungmi, Lowe, John P., Maillard, Jean-Yves ORCID: https://orcid.org/0000-0002-8617-9288, Jenkins, A. Toby A., Bull, Steven D., Sessler, Jonathan L., Yoon, Juyoung and James, Tony D. 2019. Long wavelength TCF-based fluorescent probe for the detection of alkaline phosphatase in live cells. Frontiers in Chemistry 7 , -. 10.3389/fchem.2019.00255

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Abstract

A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence “turn on” (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Publisher:	Frontiers Media
ISSN:	2296-2646
Date of First Compliant Deposit:	24 June 2019
Date of Acceptance:	1 April 2019
Last Modified:	18 Nov 2024 13:45
URI:	https://orca.cardiff.ac.uk/id/eprint/123682

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