Molecular community profiling of the bacterial microbiota associated with denture-related stomatitis

Morse, Daniel J. ORCID: https://orcid.org/0000-0002-0104-4940, Smith, Ann, Wilson, Melanie ORCID: https://orcid.org/0000-0002-8834-8268, Marsh, Lucy, White, Lewis, Posso, Raquel, Bradshaw, David J., Wei, Xiao Qing ORCID: https://orcid.org/0000-0002-6274-8503, Lewis, Michael A. O. ORCID: https://orcid.org/0000-0003-1917-0651 and Williams, David W. ORCID: https://orcid.org/0000-0002-7351-5131 2019. Molecular community profiling of the bacterial microbiota associated with denture-related stomatitis. Scientific Reports 9 , 10228. 10.1038/s41598-019-46494-0

Preview	PDF - Published Version Available under License Creative Commons Attribution. Download (3MB) | Preview
	PDF - Supplemental Material Download (251kB)

Abstract

Denture-associated stomatitis (DS) affects over two-thirds of denture-wearers. DS presents as erythema of the palatal mucosa in areas where denture-surface associated polymicrobial biofilms containing the fungus Candida albicans exist. The contribution of the oral bacterial microbiota toward the infection is unknown. Therefore, this study characterised the bacterial microbiota of sites within the oral cavity to identify potential associations with occurrence of DS. Denture-wearing patients were recruited (denture stomatitis (DS) n = 8; non-denture stomatitis (NoDS) n = 11) and the oral bacterial microbiota of the tongue, palate and denture-fitting surface was characterised using next-generation sequencing. Operational taxonomic units (OTUs) were identified to bacterial genera and species, and presence/absence and relative abundances were examined. A significant (P = 0.007) decrease in the number of OTUs and thus, diversity of the microbiota was observed in tongue samples of DS patients (vs non-DS). The microbiota of denture-fitting surfaces and palatal mucosae were similar. Large differences in the abundance of bacterial genera and species were observed at each sample site, and unique presence/absence of bacteria was noted. Presence/absence and relative abundance of specific bacteria associated with DS warrants further in vitro and in vivo evaluation, particularly as our previous work has shown C. albicans virulence factor modulation by oral bacteria.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry Biosciences Medicine
Additional Information:	This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Publisher:	Nature Publishing Group
ISSN:	2045-2322
Funders:	EPSRC
Date of First Compliant Deposit:	28 June 2019
Date of Acceptance:	27 June 2019
Last Modified:	11 Oct 2023 18:54
URI:	https://orca.cardiff.ac.uk/id/eprint/123800

Citation Data

Cited 11 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item