Comparative transcriptomics analysis of Brassica napus L. during seed maturation reveals dynamic changes in gene expression between embryos and seed coats and distinct expression profiles of Acyl-CoA-binding proteins for lipid accumulation

Liao, Pan, Woodfield, Helen K. ORCID: https://orcid.org/0000-0002-0839-6105, Harwood, John L. ORCID: https://orcid.org/0000-0003-2377-2612, Chye, Mee-len and Scofield, Simon ORCID: https://orcid.org/0000-0001-5466-314X 2019. Comparative transcriptomics analysis of Brassica napus L. during seed maturation reveals dynamic changes in gene expression between embryos and seed coats and distinct expression profiles of Acyl-CoA-binding proteins for lipid accumulation. Plant and Cell Physiology 60 (12) , pp. 2812-2825. 10.1093/pcp/pcz169

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Abstract

Production of vegetable oils is a vital agricultural resource and oilseed rape (Brassica napus) is the third most important oil crop globally. Although the regulation of lipid biosynthesis in oil seeds is still not fully defined, the acyl-CoA-binding proteins (ACBPs) have been reported to be involved in such metabolism, including oil accumulation, in several plant species. In this study, progressive changes in gene expression in embryos and seed coats at different stages of seed development were comprehensively investigated by transcriptomic analyses in B. napus, revealing dynamic changes in the expression of genes involved in lipid biosynthesis. We show that genes encoding BnACBP proteins show distinct changes in expression at different developmental stages of seed development, and show markedly different expression between embryos and seed coats. Both isoforms of the ankyrin-repeat BnACBP2 increased during the oil accumulation period of embryo development. In contrast, expression of the three most abundant isoforms of the small molecular mass BnACBP6 in embryos showed progressive reduction, despite having the highest overall expression level. In seed coats, BnACBP3, BnACBP4 and BnACBP5 expression remained constant during development while the two major isoforms of BnACBP6 increased, contrasting with the data from embryos. We conclude that genes related to fatty acid and triacylglycerol biosynthesis showing dynamic expression changes may regulate the lipid distribution in embryos and seed coats of B. napus and that BnACBP2 and BnACBP6 are potentially important for oil accumulation.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Additional Information:	This is an open access article distributed under the terms of the Creative Commons CC BY license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Publisher:	Oxford University Press
ISSN:	0032-0781
Funders:	Biotechnology and Biological Sciences Research Council
Date of First Compliant Deposit:	22 August 2019
Date of Acceptance:	18 August 2019
Last Modified:	04 May 2023 12:38
URI:	https://orca.cardiff.ac.uk/id/eprint/125067

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