Confocal laser scanning microscopy of trichomonads: Hydrogenosomes store calcium and show a membrane potential

Humphreys, Michelle, Ralphs, Jim, Durrant, Lisa and Lloyd, David ORCID: https://orcid.org/0000-0002-5656-0571 1998. Confocal laser scanning microscopy of trichomonads: Hydrogenosomes store calcium and show a membrane potential. European Journal of Protistology 34 (4) , pp. 356-362. 10.1016/S0932-4739(98)80003-5

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Abstract

Confocal laser scanning microscopy of Tritrichomonas foetus and Trichomonas vaginalis stained with Fluo-3AM a fluorescent calcium-selective probe show distinct intracellular calcium locations. The pattern of localization is comparable with the position of hydrogenosomes previously observed in these trichomonads by electron microscopy. The Ca2+-specific chelator, EGTA, sequestered Ca2+ from these Ca2+ stores when applied to living organisms. Calcium ions were also released from isolated hydrogenosomes when these organelles were diluted in vitro, but no substrate driven uptake of Ca2+ could be detected using a calcium electrode. The subcellular binding of an oxonol dye DiBAC4(3), a fluorescent membrane potential probe observed by confocal laser scanning microscopy, and confirmed by flow cytometric measurements of fluorescence emission of stained hydrogenosomes in vitro, strongly suggests the presence of a transmembrane electrochemical gradient across the membrane of this organelle.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Biosciences
Publisher:	Elsevier
ISSN:	0932-4739
Last Modified:	26 Oct 2022 08:36
URI:	https://orca.cardiff.ac.uk/id/eprint/127861

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