The regulation of propionate oxidation in Prototheca zopfii

Lloyd, D ORCID: https://orcid.org/0000-0002-5656-0571 and Venables, Susan E 1966. The regulation of propionate oxidation in Prototheca zopfii. Biochemical Journal 104 (2) , pp. 639-646. 10.1042/bj1040639

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Abstract

1. Whole cell suspensions of Prototheca zopfii grown on propionate oxidize propionate, acrylate, malonic semialdehyde and acetate immediately, whereas acetate-grown cells only oxidize acrylate or propionate rapidly after a lag of 20-30min. This adaptation to propionate is slowed down by 8-azaguanine or p-fluorophenylalanine, and is not influenced by adding an ammonium salt or an amino acid mixture. 2. The adaptation involves induction of the enzymes of beta-oxidation of propionate. 3. A small proportion (5-8%) of the activities of propionyl-CoA dehydrogenase, beta-hydroxypropionate dehydrogenase and malonic semialdehyde dehydrogenase are consistently associated with mitochondria isolated from propionate-grown cells. 4. Such mitochondria will oxidize propionyl-CoA, beta-hydroxypropionate and malonic semialdehyde, and the respiration rates with these substrates in the presence of inorganic phosphate are ADP-dependent. 5. Mitochondria from acetate-grown cells do not contain detectable activities of the enzymes of propionate oxidation.

Item Type:	Article
Date Type:	Submission
Status:	Published
Schools:	Schools > Biosciences
Publisher:	Portland Press
ISSN:	0264-6021
Last Modified:	26 Oct 2022 08:36
URI:	https://orca.cardiff.ac.uk/id/eprint/127876

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