Cell elimination strategies upon identity switch via modulation of apterous in Drosophila wing disc

Klipa, Olga and Hamaratoglu, Fisun ORCID: https://orcid.org/0000-0002-1461-9248 2019. Cell elimination strategies upon identity switch via modulation of apterous in Drosophila wing disc. PLoS Genetics 15 (12) , e1008573. 10.1371/journal.pgen.1008573

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Abstract

The ability to establish spatial organization is an essential feature of any developing tissue and is achieved through well-defined rules of cell-cell communication. Maintenance of this organization requires elimination of cells with inappropriate positional identity, a poorly understood phenomenon. Here we studied mechanisms regulating cell elimination in the context of a growing tissue, the Drosophila wing disc and its dorsal determinant Apterous. Systematic analysis of apterous mutant clones along with their twin spots shows that they are eliminated from the dorsal compartment via three different mechanisms: relocation to the ventral compartment, basal extrusion, and death, depending on the position of the clone in the wing disc. We find that basal extrusion is the main elimination mechanism in the hinge, whereas apoptosis dominates in the pouch and in the notum. In the absence of apoptosis, extrusion takes over to ensure clearance in all regions. Notably, clones in the hinge grow larger than those in the pouch, emphasizing spatial differences. Mechanistically, we find that limiting cell division within the clones does not prevent their extrusion. Indeed, even clones of one or two cells can be extruded basally, demonstrating that the clone size is not the main determinant of the elimination mechanism to be used. Overall, we revealed three elimination mechanisms and their spatial biases for preserving pattern in a growing organ.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Additional Information:	This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Publisher:	Public Library of Science
ISSN:	1553-7390
Date of First Compliant Deposit:	8 January 2020
Date of Acceptance:	17 December 2019
Last Modified:	04 May 2023 00:02
URI:	https://orca.cardiff.ac.uk/id/eprint/128356

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