Prevalence and genetic analysis of mcr-3-Positive aeromonas species from humans, retail meat, and environmental water samples

Shen, Yingbo, Xu, Chunyan, Sun, Qiaoling, Schwarz, Stefan, Ou, Yanran, Yang, Lu, Huang, Zixian, Eichhorn, Inga, Walsh, Timothy R. ORCID: https://orcid.org/0000-0003-4315-4096, Wang, Yang, Zhang, Rong and Shen, Jianzhong 2018. Prevalence and genetic analysis of mcr-3-Positive aeromonas species from humans, retail meat, and environmental water samples. Antimicrobial Agents and Chemotherapy 62 (9) , e00404-18. 10.1128/AAC.00404-18

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Abstract

The mobile colistin resistance gene mcr-3 is globally disseminated in both Enterobacteriaceae and Aeromonas species, with the latter potentially serving as a reservoir for this gene. Here, we investigated the prevalence of mcr-3 in rectal swabs from humans, in food-producing animals and their products, and in the aquatic environment, and we investigated the genetic relationships between the mcr-3-positive isolates. An enriched broth screening method was used to detect mcr-3 in samples, and species identification of isolates from positive samples was carried out by matrix-assisted laser desorption ionization–time of flight mass spectrometry and shotgun sequencing. All mcr-3-positive isolates were subjected to antimicrobial susceptibility testing, conjugation, and whole-genome sequencing. Ten Aeromonas isolates, including 2 from human rectal swabs, 1 from pork, 3 from chicken meat, and 4 from the aquatic environment, were positive for mcr-3, but only 2 showed resistance to colistin. In addition to the mcr-3 variants identified previously (the novel variants were termed mcr-3.13 to mcr-3.18), all isolates harbored mcr-3-like genes downstream of the mcr-3 variants. The MCR-3.13 to MCR-3.18 proteins exhibited only 89.2% to 96.1% amino acid identity to the original MCR-3 protein. Whole-genome sequence analysis indicated diversity within the genetic environments of mcr-3-positive Aeromonas isolates and possible transmission between different sources in China and even worldwide. Close relationships between mcr-3-positive and mcr-3-negative Aeromonas isolates suggested that mcr-3 might be common in Aeromonas species, which are not inherent hosts of mcr-3 but may act as an important reservoir of this mobile colistin resistance gene.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	American Society for Microbiology
ISSN:	0066-4804
Date of Acceptance:	18 May 2018
Last Modified:	07 Nov 2022 10:03
URI:	https://orca.cardiff.ac.uk/id/eprint/131025

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