Use of a small-scale, portable test chamber for determining the bactericidal efficacy of aerosolized glycol formulations

Rubiano, M.E., Maillard, J.-Y. ORCID: https://orcid.org/0000-0002-8617-9288, Rubino, J.R. and Ijaz, M.K. 2020. Use of a small-scale, portable test chamber for determining the bactericidal efficacy of aerosolized glycol formulations. Letters in Applied Microbiology 70 (5) , pp. 356-364. 10.1111/lam.13289

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Abstract

This study aimed to understand the efficacy and mechanisms of action of an aerosolised glycol-ethanol formulations against bacteria. We validated a small scale in-house test chamber to determine the microbicidal efficacy of four aerosolized formulations combining dipropylene glycol and ethanol against Staphylococcus aureus and Escherichia coli embedded in alginate. The aerosolised glycol/ethanol formulation decreased bacterial viability by 3 Log10 and was more efficacious than an ethanol only control formulation. Electron microscopic examination indicated extensive structural damage in both bacteria, and membrane damage was confirmed with potassium release in S. aureus and DNA release in E. coli. The development of a small test chamber facilitated the measurement of the microbicidal efficacy and experiments to understand the mechanism of action of an aerosolised microbicidal formulation.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Publisher:	Wiley
ISSN:	0266-8254
Date of First Compliant Deposit:	22 April 2020
Date of Acceptance:	20 February 2020
Last Modified:	24 Nov 2024 07:30
URI:	https://orca.cardiff.ac.uk/id/eprint/131178

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