CD40L Reverse signaling influences dendrite spine morphology and expression of PSD-95 and Rho small GTPases

Carriba, Paulina, Wyatt, Sean ORCID: https://orcid.org/0000-0002-0572-234X and Davies, Alun M. ORCID: https://orcid.org/0000-0001-5841-8176 2020. CD40L Reverse signaling influences dendrite spine morphology and expression of PSD-95 and Rho small GTPases. Frontiers in Cell and Developmental Biology 8 , 254. 10.3389/fcell.2020.00254

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Abstract

CD40-activated CD40L reverse signaling is a major physiological regulator of neural process growth from many kinds of developing neurons. Here we have investigated whether CD40L-reverse signaling also influences dendrite spine number and morphology in striatal medium spiny neurons (MSNs). Golgi preparations revealed no differences in the spine density, but because the dendrite arbors of MSNs were larger and branched in Cd40–/– mice, the total number of spines was greater in Cd40–/– mice. We also detected more mature spines compared with wild-type littermates. Western blot revealed that MSN cultures from Cd40–/– mice had significantly less PSD-95 and there were changes in RhoA/B/C and Cdc42. Immunocytochemistry revealed that PSD-95 was clustered in spines in Cd40–/– neurons compared with more diffuse labeling in Cd40+/+ neurons. Activation of CD40L-reverse signaling with CD40-Fc prevented the changes observed in Cd40–/– cultures. Our findings suggest that CD40L-reverse signaling influences dendrite spine morphology and related protein expression and distribution.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Frontiers Media
ISSN:	2296-634X
Funders:	Wellcome Trust
Date of First Compliant Deposit:	28 April 2020
Date of Acceptance:	25 March 2020
Last Modified:	05 May 2023 15:56
URI:	https://orca.cardiff.ac.uk/id/eprint/131282

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