Uncovering the chemistry of C–C bond formation in C-nucleoside biosynthesis: crystal structure of a C-glycoside synthase/PRPP complex

Gao, Sisi, Radadiya, Ashish ORCID: https://orcid.org/0000-0001-7348-1755, Li, Wenbo, Liu, Huanting, Zhu, Wen, de Crécy-Lagard, Valérie, Richards, Nigel G. J. ORCID: https://orcid.org/0000-0002-0375-0881 and Naismith, James H. 2020. Uncovering the chemistry of C–C bond formation in C-nucleoside biosynthesis: crystal structure of a C-glycoside synthase/PRPP complex. Chemical Communications 56 (55) , pp. 7617-7620. 10.1039/D0CC02834G

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Abstract

The enzyme ForT catalyzes C–C bond formation between 5′-phosphoribosyl-1′-pyrophosphate (PRPP) and 4-amino-1H-pyrazole-3,5-dicarboxylate to make a key intermediate in the biosynthesis of formycin A 5′-phosphate by Streptomyces kaniharaensis. We report the 2.5 Å resolution structure of the ForT/PRPP complex and locate active site residues critical for PRPP recognition and catalysis.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Chemistry Advanced Research Computing @ Cardiff (ARCCA)
Publisher:	Royal Society of Chemistry
ISSN:	1359-7345
Funders:	BBSRC
Date of First Compliant Deposit:	10 June 2020
Date of Acceptance:	14 May 2020
Last Modified:	24 Feb 2024 10:34
URI:	https://orca.cardiff.ac.uk/id/eprint/132329

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