Sedgwick, Adam C., Gardiner, Jordan E., Kim, Gyoungmi, Yevglevskis, Maksims, Lloyd, Matthew D., Jenkins, A. Toby A., Bull, Steven D., Yoon, Juyoung and James, Tony D.
2018.
Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols.
Chemical Communications
54
(38)
, pp. 4786-4789.
10.1039/C8CC01661E
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Abstract
Two ‘turn on’ TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found for TCFCl-GSH and a clear ‘turn on’ with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Chemistry |
Publisher: | Royal Society of Chemistry |
ISSN: | 1359-7345 |
Date of First Compliant Deposit: | 13 July 2020 |
Date of Acceptance: | 16 April 2018 |
Last Modified: | 06 May 2023 04:27 |
URI: | https://orca.cardiff.ac.uk/id/eprint/133374 |
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