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Multiple spatially specific enhancers are required to reconstruct the pattern of Hox-2.6 gene expression

Whiting, J., Marshall, H., Cook, M., Krumlauf, R., Rigby, P. W., Stott, D. and Allemann, Rudolf Konrad ORCID: 1991. Multiple spatially specific enhancers are required to reconstruct the pattern of Hox-2.6 gene expression. Genes & Development 5 (11) , pp. 2048-2059. 10.1101/gad.5.11.2048

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Murine Hox genes are organized into four clusters that share many features with the homeotic clusters of Drosophila. This evolutionary conservation and the clear relationships between the position of a gene within a cluster and its expression pattern have led to the suggestion that the structure of the cluster is essential for proper regulation. Using a Hox-2.6-lacZ reporter gene in transgenic mice we have shown that the overall expression pattern of the endogenous Hox-2.6 gene can be reconstructed when it is isolated from the complex. The transgene was expressed in the proper tissues, with the correct spatial distribution and temporal pattern. Furthermore, direct comparison by in situ hybridization revealed that the levels of transgene expression are similar to those of the endogenous gene. This has allowed us to define three elements that regulate particular aspects of the Hox-2.6 pattern, two of which act as spatially specific enhancers. One enhancer, region A, directed expression only in the neural tube, whereas the other, region C, specified the majority of the Hox-2.6 pattern. Both were also capable of imposing the correct boundaries of expression on heterologous promoters. The definition of such elements will allow the characterization of the trans-acting factors that mediate spatial regulation in the mammalian embryo.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Cardiff Catalysis Institute (CCI)
Subjects: Q Science > QD Chemistry
Publisher: Cold Spring Harbor Laboratory Press
ISSN: 0890-9369
Date of First Compliant Deposit: 30 March 2016
Last Modified: 04 May 2023 21:30

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