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X-ray diffraction imaging of corneal ultrastructure

Meek, Keith M. ORCID: https://orcid.org/0000-0002-9948-7538, Quantock, Andrew J. ORCID: https://orcid.org/0000-0002-2484-3120, Hayes, Sally ORCID: https://orcid.org/0000-0001-8550-0108 and Bell, James ORCID: https://orcid.org/0000-0001-8371-9851 2020. X-ray diffraction imaging of corneal ultrastructure. Ahearne, Mark, ed. Corneal Regeneration: Methods and Protocols, Vol. 2145. Methods in Molecular Biology, New York, NY, USA: Humana, pp. 231-247. (10.1007/978-1-0716-0599-8_16)

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Abstract

X-ray scattering enables the structure of collagen-rich tissues, such as the cornea, to be examined at both the molecular and fibrillar level. The high-intensity X-rays available at synchrotron radiation sources, coupled with minimal sample preparation requirements, facilitates the rapid generation of high-quality X-ray scattering data from corneal tissue at a close-to-physiological state of hydration. Analysis of resulting X-ray scatter patterns allows one to quantify numerous structural parameters relating to the average diameter, lateral arrangement and alignment of collagen fibrils within the cornea, as well as the axial and lateral arrangements of collagen molecules within the fibrils. Here we describe the typical experimental setup and considerations involved in the collection of X-ray scattering data from corneal tissue.

Item Type: Book Section
Date Type: Published Online
Status: Published
Schools: Optometry and Vision Sciences
Publisher: Humana
ISBN: 9781071605981
Last Modified: 27 Nov 2022 12:04
URI: https://orca.cardiff.ac.uk/id/eprint/134651

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