Reduced Susceptibility of Moritella profunda Dihydrofolate Reductase to Trimethoprim is Not Due to Glutamate 28

Loveridge, Edric Joel, Dawson, William Michael, Evans, Rhiannon Mari, Sobolewska, Anna and Allemann, Rudolf Konrad ORCID: https://orcid.org/0000-0002-1323-8830 2011. Reduced Susceptibility of Moritella profunda Dihydrofolate Reductase to Trimethoprim is Not Due to Glutamate 28. The Protein Journal 30 (8) , pp. 546-548. 10.1007/s10930-011-9361-x

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Abstract

The E28D variant of dihydrofolate reductase from Moritella profunda was generated and found to have the same K i (within error) for the competitive inhibitor trimethoprim as the wild type enzyme. Contrary to a previous claim in the literature, Glu 28 is therefore not the cause of the reduced affinity for trimethoprim relative to dihydrofolate reductase from Escherichia coli.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Cardiff Catalysis Institute (CCI) Chemistry
Subjects:	Q Science > QD Chemistry
Uncontrolled Keywords:	Dihydrofolate reductase – Moritella profunda – Trimethoprim – Inhibition
Publisher:	Springer
ISSN:	1572-3887
Funders:	EPSRC, EU
Last Modified:	18 Oct 2022 13:20
URI:	https://orca.cardiff.ac.uk/id/eprint/13504

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