Reclassification of the biocontrol agents Bacillus subtilis BY-2 and Tu-100 as Bacillus velezensis and insights into the genomic and specialised metabolite diversity of the species

Mullins, Alex J. ORCID: https://orcid.org/0000-0001-5804-9008, Li, Yinshui, Qin, Lu, Hu, Xiaojia, Xie, Lihua, Gu, Chiming, Mahenthiralingam, Eshwar ORCID: https://orcid.org/0000-0001-9014-3790, Liao, Xing and Webster, Gordon ORCID: https://orcid.org/0000-0002-9530-7835 2020. Reclassification of the biocontrol agents Bacillus subtilis BY-2 and Tu-100 as Bacillus velezensis and insights into the genomic and specialised metabolite diversity of the species. Microbiology 166 (12) , pp. 1121-1128. 10.1099/mic.0.000986

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Abstract

The genomes of two historical Bacillus species strains isolated from the roots of oilseed rape and used routinely in PR China as biocontrol agents to suppress Sclerotinia disease were sequenced. Average nucleotide identity (ANI) and digital DNA–DNA hybridization analyses demonstrated that they were originally misclassified as Bacillus subtilis and now belong to the bacterial species Bacillus velezensis . A broader ANI analysis of available Bacillus genomes identified 292 B. velezensis genomes that were then subjected to core gene analysis and phylogenomics. Prediction and dereplication of specialized metabolite biosynthetic gene clusters (BGCs) defined the prevalence of multiple antimicrobial-associated BGCs and highlighted the natural product potential of B. velezensis . By defining the core and accessory antimicrobial biosynthetic capacity of the species, we offer an in-depth understanding of B. velezensis natural product capacity to facilitate the selection and testing of B. velezensis strains for use as biological control agents.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Additional Information:	This is an open-access article distributed under the terms of the Creative Commons Attribution License
Publisher:	Microbiology Society
ISSN:	1350-0872
Date of First Compliant Deposit:	27 October 2020
Date of Acceptance:	10 October 2020
Last Modified:	15 Nov 2024 22:24
URI:	https://orca.cardiff.ac.uk/id/eprint/135988

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