Direct characterization of circulating DNA in blood plasma using μLAS technology

Malbec, R., Chami, B., Ngo, H. H. T., Didelot, A., Garlan, F., Garrigou, S., Taly, V., Aeschbach, Lorene, Trofimenko, Evgeniya, Dion, Vincent ORCID: https://orcid.org/0000-0003-4953-7637, Boutonnet-Rodat, A., Ginot, F. and Bancaud, A. 2018. Direct characterization of circulating DNA in blood plasma using μLAS technology. Presented at: IEEE International Electron Devices Meeting (IEDM 2017), San Francisco, CA, USA, 2-6 December 2017. 2017 IEEE International Electron Devices Meeting (IEDM). IEEE, 26.5.1-26.5.4. 10.1109/IEDM.2017.8268465

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Abstract

Circulating cell-free DNA (cfDNA) is a powerful cancer biomarker for establishing targeted therapies or monitoring patients' treatment. However, current cfDNA characterization is severely limited by its low concentration, requiring the extensive use of amplification techniques. Here we report that the μLAS technology allows us to quantitatively characterize the size distribution of purified cfDNA in a few minutes, even when its concentration is as low as 1 pg/μL. Moreover, we show that DNA profiles can be directly measured in blood plasma with a minimal conditioning process to speed up considerably speed up the cfDNA analytical chain.

Item Type:	Conference or Workshop Item (Paper)
Date Type:	Published Online
Status:	Published
Schools:	Medicine
Publisher:	IEEE
ISBN:	9781538635599
ISSN:	2156-017X
Date of First Compliant Deposit:	28 October 2020
Date of Acceptance:	3 November 2017
Last Modified:	09 Nov 2022 09:31
URI:	https://orca.cardiff.ac.uk/id/eprint/136013

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