Multimerization of the Dnmt3a DNA methyltransferase and its functional implications

Jeltsch, Albert and Jurkowska, Renata Z. ORCID: https://orcid.org/0000-0002-4507-2222 2013. Multimerization of the Dnmt3a DNA methyltransferase and its functional implications. Progress in Molecular Biology and Translational Science 117 , pp. 445-464. 10.1016/B978-0-12-386931-9.00016-7

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Abstract

The Dnmt3a DNA cytosine-C5 methyltransferase has been recently shown to exhibit a complex oligomerization and multimerization potential, the structural basis and functional implications of which will be the subject of this contribution. The enzyme forms a linear heterotetramer with Dnmt3L, in which the interaction of Dnmt3a and 3L stimulates the catalytic activity of Dnmt3a. Isolated Dnmt3a forms protein filaments that bind to several DNA molecules oriented in parallel, which plays an essential role in the location of the enzyme to heterochromatin. Dnmt3L disrupts Dnmt3a protein filaments and leads to a redistribution of the enzyme in cells toward euchromatin. Finally, Dnmt3a complexes and Dnmt3a/3L heterotetramers cooperatively multimerize on DNA forming protein–DNA filaments. This leads to a preference of the enzyme for periodic methylation of DNA and supports its heterochromatic localization.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Academic Press
ISSN:	1877-1173
Last Modified:	09 Nov 2022 10:22
URI:	https://orca.cardiff.ac.uk/id/eprint/139285

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