Asgharzade, Samira, Sewell, Robert, Rabiei, Zahra, Forouzanfar, Fatemeh, Sheikhshabani, Sedigheh and Rafieian-Kopaei, Mahmoud
2020.
Hydroalcoholic extract of anchusa italica protects global cerebral ischemia-reperfusion injury via a nitrergic mechanism.
Basic and Clinical Neuroscience
11
(3)
, pp. 323-332.
10.32598/bcn.11.2.1665.2
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Abstract
Introduction: In stroke models, Inducible Nitric Oxide Synthase (iNOS) expression initiates cellular toxicity due to excessive Nitric Oxide (NO) generation. Anchusa italica is a medicinal herb with anti-inflammatory, antioxidant and neuroprotective properties. This study evaluated the antioxidant activity and NOS mRNA expression of the Hydroalcoholic Extract Of Anchusa Italica (HEAI) in an experimental stroke model in rats. Methods: The stroke model was induced by bilateral occlusion of both common carotid arteries for 60 min. Twenty-four hours after surgery, HEAI (50 and 100 mg/kg i.p.) was injected daily for 10 consecutive days. mRNA expression levels of NOS subtypes and hippocampal Brain-Derived Neurotrophic Factor (BDNF) were studied using real-time PCR. Besides, hippocampal tissue plus serum concentrations of NO and Malondialdehyde (MDA) were measured. Results: HEAI decreased MDA in both serum and hippocampal tissue and also reduced serum NO levels. Additionally, in the HEAI-treated groups, a down-regulation of iNOS mRNA expression, and an up-regulation of BDNF mRNA expression were observed. Conclusion: The results indicated that the administration of HEAI even after the onset of ischemia protects the brain from free radical injury and inflammation via a down-regulation of iNOS expression inhibiting NO production and an up-regulation of BDNF mRNA.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Pharmacy |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology |
| Uncontrolled Keywords: | Anchusa italica, Ischemiareperfusion, Nitric oxide, Stroke |
| Publisher: | Tehran University of Medical Sciences |
| ISSN: | 2008-126X |
| Date of First Compliant Deposit: | 18 March 2021 |
| Date of Acceptance: | 20 July 2019 |
| Last Modified: | 03 May 2023 01:30 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/139870 |
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