Hydroalcoholic extract of anchusa italica protects global cerebral ischemia-reperfusion injury via a nitrergic mechanism

Asgharzade, Samira, Sewell, Robert, Rabiei, Zahra, Forouzanfar, Fatemeh, Sheikhshabani, Sedigheh and Rafieian-Kopaei, Mahmoud 2020. Hydroalcoholic extract of anchusa italica protects global cerebral ischemia-reperfusion injury via a nitrergic mechanism. Basic and Clinical Neuroscience 11 (3) , pp. 323-332. 10.32598/bcn.11.2.1665.2

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Abstract

Introduction: In stroke models, Inducible Nitric Oxide Synthase (iNOS) expression initiates cellular toxicity due to excessive Nitric Oxide (NO) generation. Anchusa italica is a medicinal herb with anti-inflammatory, antioxidant and neuroprotective properties. This study evaluated the antioxidant activity and NOS mRNA expression of the Hydroalcoholic Extract Of Anchusa Italica (HEAI) in an experimental stroke model in rats. Methods: The stroke model was induced by bilateral occlusion of both common carotid arteries for 60 min. Twenty-four hours after surgery, HEAI (50 and 100 mg/kg i.p.) was injected daily for 10 consecutive days. mRNA expression levels of NOS subtypes and hippocampal Brain-Derived Neurotrophic Factor (BDNF) were studied using real-time PCR. Besides, hippocampal tissue plus serum concentrations of NO and Malondialdehyde (MDA) were measured. Results: HEAI decreased MDA in both serum and hippocampal tissue and also reduced serum NO levels. Additionally, in the HEAI-treated groups, a down-regulation of iNOS mRNA expression, and an up-regulation of BDNF mRNA expression were observed. Conclusion: The results indicated that the administration of HEAI even after the onset of ischemia protects the brain from free radical injury and inflammation via a down-regulation of iNOS expression inhibiting NO production and an up-regulation of BDNF mRNA.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords:	Anchusa italica, Ischemiareperfusion, Nitric oxide, Stroke
Publisher:	Tehran University of Medical Sciences
ISSN:	2008-126X
Date of First Compliant Deposit:	18 March 2021
Date of Acceptance:	20 July 2019
Last Modified:	03 May 2023 01:30
URI:	https://orca.cardiff.ac.uk/id/eprint/139870

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