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Asparaginyl endopeptidases: enzymology, applications and limitations

Tang, Tsz Man Simon Louis and Luk, Louis Y. P. ORCID: 2021. Asparaginyl endopeptidases: enzymology, applications and limitations. Organic and Biomolecular Chemistry 19 (23) , pp. 5048-5062. 10.1039/D1OB00608H

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Asparaginyl endopeptidases (AEP) are cysteine proteases found in mammalian and plant cells. Several AEP isoforms from plant species were found to exhibit transpeptidase activity which is integral for the key head-to-tail cyclisation reaction during the biosynthesis of cyclotides. Since many plant AEPs exhibit excellent enzyme kinetics for peptide ligation via a relatively short substrate recognition sequence, they have become appealing tools for peptide and protein modification. In this review, research focused on the enzymology of AEPs and their applications in polypeptide cyclisation and labelling will be presented. Importantly, the limitations of using AEPs and opportunities for future research and innovation will also be discussed.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Additional Information: This article is licensed under a Creative Commons Attribution 3.0 Unported Licence
Publisher: Royal Society of Chemistry
ISSN: 1477-0520
Funders: BBSRC and UKRI
Date of First Compliant Deposit: 13 May 2021
Date of Acceptance: 12 May 2021
Last Modified: 19 May 2023 02:18

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