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Extracellular matrix deposition and remodeling after corneal alkali burn in mice

Mutoji, Kazadi N., Sun, Mingxia, Elliott, Garrett, Moreno, Isabel Y., Hughes, Clare ORCID:, Gesteira, Tarsis F. and Coulson-Thomas, Vivien J. 2021. Extracellular matrix deposition and remodeling after corneal alkali burn in mice. International Journal of Molecular Sciences 22 (11) , 5708. 10.3390/ijms22115708

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Corneal transparency relies on the precise arrangement and orientation of collagen fibrils, made of mostly Type I and V collagen fibrils and proteoglycans (PGs). PGs are essential for correct collagen fibrillogenesis and maintaining corneal homeostasis. We investigated the spatial and temporal distribution of glycosaminoglycans (GAGs) and PGs after a chemical injury. The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas. The expression profile and corneal distribution of CS/DSPGs and keratan sulfate (KS) PGs were also analyzed. We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS. Expression of the CSPGs biglycan and versican was increased after AB, while decorin expression was decreased. We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression. No significant changes in HS composition were noted after AB. Taken together, our study reveals significant changes in the composition of the extracellular matrix following a corneal chemical injury.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Additional Information: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// 4.0/
Publisher: MDPI
ISSN: 1422-0067
Date of First Compliant Deposit: 8 June 2021
Date of Acceptance: 25 May 2021
Last Modified: 05 May 2023 22:36

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