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DNA-directed assembly of carbon nanotube-protein hybrids

Freeley, Mark, Gwyther, Rebecca E. A., Jones, D. Dafydd ORCID: and Palma, Matteo 2021. DNA-directed assembly of carbon nanotube-protein hybrids. Biomolecules 11 (7) , 955. 10.3390/biom11070955

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Here, we report the controlled assembly of SWCNT–GFP hybrids employing DNA as a linker. Two distinct, enriched SWCNTs chiralities, (6,5), (7,6), and an unsorted SWCNT solution, were selectively functionalized with DNA and hybridized to a complementary GFPDNA conjugate. Atomic force microscopy images confirmed that GFP attachment occurred predominantly at the terminal ends of the nanotubes, as designed. The electronic coupling of the proteins to the nanotubes was confirmed via in-solution fluorescence spectroscopy, that revealed an increase in the emission intensity of GFP when linked to the CNTs.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Advanced Research Computing @ Cardiff (ARCCA)
Publisher: MDPI
Date of First Compliant Deposit: 5 July 2021
Date of Acceptance: 18 June 2021
Last Modified: 21 May 2023 00:41

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