Biofunctionalisation of gallium arsenide with neutravidin

Santos Gomes, Bárbara, Morgan, David J. ORCID: https://orcid.org/0000-0002-6571-5731, Langbein, Wolfgang ORCID: https://orcid.org/0000-0001-9786-1023, Borri, Paola ORCID: https://orcid.org/0000-0002-7873-3314 and Masia, Francesco ORCID: https://orcid.org/0000-0003-4958-410X 2022. Biofunctionalisation of gallium arsenide with neutravidin. Journal of Colloid and Interface Science 608 (P3) , pp. 2399-2406. 10.1016/j.jcis.2021.10.135

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Abstract

Gallium arsenide (GaAs) is a promising candidate as a platform for optical biosensing devices due to its enabling optoelectronic properties. However, the biofunctionalisation of the GaAs surface has not received much attention compared to gold, carbon and silicon surfaces. Here we report a study presenting a physicochemical surface characterisation of the GaAs surface along the functionalisation with a high-affinity bioconjugation pair widely explored in the life sciences – biotin and neutravidin. Combined X-ray photoelectron spectroscopy (XPS), wettability measurements and spectroscopic ellipsometry were used for a reliable characterisation of the surface functionalisation process. The results suggest that a film with a thickness lower than 10 nm was formed, with a neutravidin to biotin ratio of 1:25 on the GaAs surface. Reduction of non-specific binding of the protein to the surface was achieved by optimising the protein buffer and rinsing steps. This study shows the feasibility of using GaAs as a platform for specific biomolecular recognition, paving the way to a new generation of optoelectronic biosensors.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Chemistry Physics and Astronomy Biosciences Cardiff Catalysis Institute (CCI)
Publisher:	Elsevier
ISSN:	0021-9797
Date of First Compliant Deposit:	8 November 2021
Date of Acceptance:	24 October 2021
Last Modified:	11 Nov 2024 19:45
URI:	https://orca.cardiff.ac.uk/id/eprint/145374

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