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Characterization of DNA methylomic signatures in induced pluripotent stem cells during neuronal differentiation

Imm, Jennifer, Pishva, Ehsan, Ali, Muhammadd, Kerrigan, Talitha L., Jeffries, Aaron, Burrage, Joe, Glaab, Enrico, Cope, Emma L., Jones, Kimberley M., Allen, Nicholas D. ORCID: and Lunnon, Katie 2021. Characterization of DNA methylomic signatures in induced pluripotent stem cells during neuronal differentiation. Frontiers in Cell and Developmental Biology 9 , 647981. 10.3389/fcell.2021.647981

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In development, differentiation from a pluripotent state results in global epigenetic changes, although the extent to which this occurs in induced pluripotent stem cell-based neuronal models has not been extensively characterized. In the present study, induced pluripotent stem cell colonies (33Qn1 line) were differentiated and collected at four time-points, with DNA methylation assessed using the Illumina Infinium Human Methylation EPIC BeadChip array. Dynamic changes in DNA methylation occurring during differentiation were investigated using a data-driven trajectory inference method. We identified a large number of Bonferroni-significant loci that showed progressive alterations in DNA methylation during neuronal differentiation. A gene–gene interaction network analysis identified 60 densely connected genes that were influential in the differentiation of neurons, with STAT3 being the gene with the highest connectivity.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Biosciences
Additional Information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
Publisher: Frontiers Media
ISSN: 2296-634X
Date of First Compliant Deposit: 13 January 2022
Date of Acceptance: 14 May 2021
Last Modified: 13 May 2023 21:05

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