Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Host immunomodulatory lipids created by symbionts from dietary amino acids

Oh, Sungwhan F., Praveena, T., Song, Heebum, Yoo, Ji-Sun, Jung, Da-Jung, Erturk-Hasdemir, Deniz, Hwang, Yoon Soo, Lee, ChangWon C., Le Nours, Jérôme, Kim, Hyunsoo, Lee, Jesang, Blumberg, Richard S., Rossjohn, Jamie, Park, Seung Bum and Kasper, Dennis L. 2021. Host immunomodulatory lipids created by symbionts from dietary amino acids. Nature 600 (7888) , 302–307. 10.1038/s41586-021-04083-0

Full text not available from this repository.

Abstract

Small molecules derived from symbiotic microbiota critically contribute to intestinal immune maturation and regulation1. However, little is known about the molecular mechanisms that control immune development in the host–microbiota environment. Here, using a targeted lipidomic analysis and synthetic approach, we carried out a multifaceted investigation of immunomodulatory α-galactosylceramides from the human symbiont Bacteroides fragilis (BfaGCs). The characteristic terminal branching of BfaGCs is the result of incorporation of branched-chain amino acids taken up in the host gut by B. fragilis. A B. fragilis knockout strain that cannot metabolize branched-chain amino acids showed reduced branching in BfaGCs, and mice monocolonized with this mutant strain had impaired colonic natural killer T (NKT) cell regulation, implying structure-specific immunomodulatory activity. The sphinganine chain branching of BfaGCs is a critical determinant of NKT cell activation, which induces specific immunomodulatory gene expression signatures and effector functions. Co-crystal structure and affinity analyses of CD1d–BfaGC–NKT cell receptor complexes confirmed the interaction of BfaGCs as CD1d-restricted ligands. We present a structural and molecular-level paradigm of immunomodulatory control by interactions of endobiotic metabolites with diet, microbiota and the immune system.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Nature Research
ISSN: 0028-0836
Date of First Compliant Deposit: 26 January 2022
Date of Acceptance: 28 September 2021
Last Modified: 01 Feb 2022 12:15
URI: https://orca.cardiff.ac.uk/id/eprint/146954

Citation Data

Cited 8 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item