Behavioural and electrophysiological responses of female Anopheles gambiae mosquitoes to volatiles from a mango bait

Meza, Felician C., Roberts, Joe M., Sobhy, Islam S. ORCID: https://orcid.org/0000-0003-4984-1823, Okumu, Fredros O., Tripet, Frederic and Bruce, Toby J. A. 2020. Behavioural and electrophysiological responses of female Anopheles gambiae mosquitoes to volatiles from a mango bait. Journal of Chemical Ecology 46 (4) , pp. 387-396. 10.1007/s10886-020-01172-8

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Abstract

Attractive Toxic Sugar Baits (ATSB) are used in a “lure-and-kill” approach for management of the malaria vector Anopheles gambiae, but the active chemicals were previously unknown. Here we collected volatiles from a mango, Mangifera indica, juice bait which is used in ATSBs in Tanzania and tested mosquito responses. In a Y-tube olfactometer, female mosquitoes were attracted to the mango volatiles collected 24–48 h, 48–72 h and 72–96 h after preparing the bait but volatiles collected at 96–120 h were no longer attractive. Volatile analysis revealed emission of 23 compounds in different chemical classes including alcohols, aldehydes, alkanes, benzenoids, monoterpenes, sesquiterpenes and oxygenated terpenes. Coupled GC-electroantennogram (GC-EAG) recordings from the antennae of An. gambiae showed robust responses to 4 compounds: humulene, (E)-caryophyllene, terpinolene and myrcene. In olfactometer bioassays, mosquitoes were attracted to humulene and terpinolene. (E)-caryophyllene was marginally attractive while myrcene elicited an avoidance response with female mosquitoes. A blend of humulene, (E)-caryophyllene and terpinolene was highly attractive to females (P < 0.001) when tested against a solvent blank. Furthermore, there was no preference when this synthetic blend was offered as a choice against the natural sample. Our study has identified the key compounds from mango juice baits that attract An. gambiae and this information may help to improve the ATSBs currently used against malaria vectors.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Additional Information:	This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
Publisher:	Springer
ISSN:	0098-0331
Date of First Compliant Deposit:	22 February 2022
Date of Acceptance:	16 March 2020
Last Modified:	04 May 2023 20:29
URI:	https://orca.cardiff.ac.uk/id/eprint/147636

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