Kidd, Emma Jane  ORCID: https://orcid.org/0000-0001-5507-1170, Michel, A. D. and Humphrey, P. P. A.
1995.
Autoradiographic distribution of [3H]l-NG-Nitro-arginine binding in rat brain.
Neuropharmacology
34
(1)
, pp. 63-73.
10.1016/0028-3908(94)00132-C
|
Abstract
The distribution of nitric oxide synthase (NOS), the enzyme which produces nitric oxide, has previously been studied in the rat central nervous system (CNS) using the NADPH-diaphorase technique and anti-NOS antibodies. However, the former method may not always be selective for NOS while the latter is not quantitative. Therefore a selective, quantifiable method would be desirable. l-NG-Nitro-arginine, an inhibitor of NOS, is available in a tritiated form which we have shown to bind to NOS. We have now examined the regional distribution of [3H]l-NG-nitro-argininebinding in the rat CNS using autoradiography. [3H]l-NG-nitro-arginine specific binding was seen in a number of brain regions with the highest levels in the accessory olfactory bulb, the amygdaloid complex, the Islands of Calleja and the cerebellum. This regional distribution of [3H]l-NG-nitro-argininebinding sites in the rat CNS was, in general, similar to that seen with the NADPH-diaphorase method and anti-NOS antibodies, consistent with the view that all three methods identify NOS in the CNS. Thus, [3H]l-NG-nitro-arginine appears to be a useful radioligand for studying the distribution of NOS in the CNS as its binding is quantifiable and apparently selective for NOS.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Pharmacy |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology |
| Uncontrolled Keywords: | Nitric oxide synthase; rat central nervous system; [3H]l-NG-nitro-arginine; autoradiography; regional distribution; quantification |
| Publisher: | Elsevier |
| ISSN: | 0028-3908 |
| Last Modified: | 18 Oct 2022 13:39 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/14766 |
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