Autoradiographic distribution of [3H]l-NG-Nitro-arginine binding in rat brain

Kidd, Emma Jane ORCID: https://orcid.org/0000-0001-5507-1170, Michel, A. D. and Humphrey, P. P. A. 1995. Autoradiographic distribution of [3H]l-NG-Nitro-arginine binding in rat brain. Neuropharmacology 34 (1) , pp. 63-73. 10.1016/0028-3908(94)00132-C

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Abstract

The distribution of nitric oxide synthase (NOS), the enzyme which produces nitric oxide, has previously been studied in the rat central nervous system (CNS) using the NADPH-diaphorase technique and anti-NOS antibodies. However, the former method may not always be selective for NOS while the latter is not quantitative. Therefore a selective, quantifiable method would be desirable. l-NG-Nitro-arginine, an inhibitor of NOS, is available in a tritiated form which we have shown to bind to NOS. We have now examined the regional distribution of [3H]l-NG-nitro-argininebinding in the rat CNS using autoradiography. [3H]l-NG-nitro-arginine specific binding was seen in a number of brain regions with the highest levels in the accessory olfactory bulb, the amygdaloid complex, the Islands of Calleja and the cerebellum. This regional distribution of [3H]l-NG-nitro-argininebinding sites in the rat CNS was, in general, similar to that seen with the NADPH-diaphorase method and anti-NOS antibodies, consistent with the view that all three methods identify NOS in the CNS. Thus, [3H]l-NG-nitro-arginine appears to be a useful radioligand for studying the distribution of NOS in the CNS as its binding is quantifiable and apparently selective for NOS.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Pharmacy
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords:	Nitric oxide synthase; rat central nervous system; [3H]l-NG-nitro-arginine; autoradiography; regional distribution; quantification
Publisher:	Elsevier
ISSN:	0028-3908
Last Modified:	18 Oct 2022 13:39
URI:	https://orca.cardiff.ac.uk/id/eprint/14766

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