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Flow cytometry analysis of hematopoietic stem/progenitor cells and mature blood cell subsets in atherosclerosis

Almotiri, Alhomidi, Abdelfattah, Ali and Rodrigues, Neil P. 2022. Flow cytometry analysis of hematopoietic stem/progenitor cells and mature blood cell subsets in atherosclerosis. In: Ramji, Dipak ed. Atherosclerosis, Vol. 2419. Methods in Molecular Biology, Springer, pp. 583-595. (10.1007/978-1-0716-1924-7_36)

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Abstract

Advancing age causes physiologic decline in tissue function. In the hematopoietic system this manifests as a progressive reduction in blood or immune cell function and clonal hematopoiesis, where a mutated hematopoietic stem cell can dominate blood cell production and confer an increased propensity for myeloid malignancy. In the aging cardiovascular system, atherosclerosis causes an inflammatory cell- driven accumulation of lipid-derived plaques in major arteries which constrains blood flow and can lead to myocardial infarction and stroke. Clonal hematopoiesis in the elderly has recently been associated with a substantially increased risk of atherosclerosis-related cardiovascular disease. However, the direct association between deregulated hematopoiesis in clonal hematopoiesis and atherosclerosis is poorly defined. Herein, we describe a flow cytometry method to prospectively analyze the crucial hematopoietic stem/progenitor, inflammatory and lymphoid cell participants in atherosclerosis. This analysis can be applied to decipher the complex relationship between hematopoietic cell types involved in clonal hematopoiesis and atherosclerosis in mouse models.

Item Type: Book Section
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Springer
ISBN: 9781071619247
ISSN: 1064-3745
Last Modified: 14 Mar 2022 11:39
URI: https://orca.cardiff.ac.uk/id/eprint/148114

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