Degradation of the resin-dentin bonds after simulated and inhibited cariogenic challenge in an in situ model

Reinke, S. M. G., De Campos Lawder, J. A., Divardin, S., Raggio, D. ORCID: https://orcid.org/0000-0002-0048-2068, Reis, A. and Loguercio, A. D. 2012. Degradation of the resin-dentin bonds after simulated and inhibited cariogenic challenge in an in situ model. Journal of Biomedical Materials Research - Part B Applied Biomaterials 100 B (6) , pp. 1466-1471. 10.1002/jbm.b.32708

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Abstract

The aim of this study was to evaluate the resin–dentin bonds of two simplified etch-and-rinse adhesive after simulated cariogenic and inhibited cariogenic challenge in situ. Dental cavities (4 mm wide, 4 mm long, and 1.5 mm deep) were prepared in 60 bovine teeth with enamel margins. Restorations were bonded with either adhesive Adper Single Bond 2 (3MESPE) or Optibond Solo Plus (Kerr). Forty restorations were included in an intra-oral palatal appliance that was used for 10 adult volunteers while the remaining 20 dental blocks were not submitted to any cariogenic challenge [NC group] and tested immediately. For the simulated cariogenic challenge [C+DA], each volunteer dropped 20% sucrose solution onto all blocks four times a day during 14 days and distilled water twice a day. In the inhibited cariogenic challenge group [C + FA], the same procedure was done, but slurry of fluoride dentifrice (1.100 ppm) was applied instead of water. The restored bovine blocks were sectioned to obtain a slice for cross-sectional Vickers microhardness evaluation and resin–dentin bonded sticks (0.8 mm2) for resin–dentin microtensile evaluation. Data were evaluated by two-way ANOVA and Tukey's tests (α = 0.05). Statistically lower microhardness values and degradation of the resin–dentin bonds were only found in the C + DW group for both adhesives. The in situ model seems to be a suitable short-term methodology to investigate the degradation of the resin–dentin bonds under a more realistic condition.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Last Modified:	10 Nov 2022 10:57
URI:	https://orca.cardiff.ac.uk/id/eprint/148785

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