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Synaptic vesicle binding of α-synuclein is modulated by β- and γ-synucleins

Carnazza, Kathryn E., Komer, Lauren E., Xie, Ying Xue, Pineda, André, Briano, Juan Antonio, Gao, Virginia, Na, Yoonmi, Ramlall, Trudy, Buchman, Vladimir L. ORCID:, Eliezer, David, Sharma, Manu and Burré, Jacqueline 2022. Synaptic vesicle binding of α-synuclein is modulated by β- and γ-synucleins. Cell Reports 39 (2) , 110675. 10.1016/j.celrep.2022.110675

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α-synuclein, β-synuclein, and γ-synuclein are abundantly expressed proteins in the vertebrate nervous system. α-synuclein functions in neurotransmitter release by binding to and clustering synaptic vesicles and chaperoning SNARE-complex assembly. Pathologically, aggregates originating from soluble pools of α-synuclein are deposited into Lewy bodies in Parkinson’s disease and related synucleinopathies. The functions of β-synuclein and γ-synuclein in presynaptic terminals remain poorly studied. Using in vitro liposome binding studies, circular dichroism spectroscopy, immunoprecipitation, and fluorescence resonance energy transfer (FRET) experiments on isolated synaptic vesicles in combination with subcellular fractionation of brains from synuclein mouse models, we show that β-synuclein and γ-synuclein have a reduced affinity toward synaptic vesicles compared with α-synuclein, and that heteromerization of β-synuclein or γ-synuclein with α-synuclein results in reduced synaptic vesicle binding of α-synuclein in a concentration-dependent manner. Our data suggest that β-synuclein and γ-synuclein are modulators of synaptic vesicle binding of α-synuclein and thereby reduce α-synuclein’s physiological activity at the neuronal synapse.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Additional Information: Creative Commons Attribution – NonCommercial – NoDerivs (CC BY-NC-ND 4.0)
Publisher: Cell Press
ISSN: 2211-1247
Date of First Compliant Deposit: 1 June 2022
Date of Acceptance: 22 March 2022
Last Modified: 10 Nov 2022 11:21

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